Real-time Polymerase Chain Reaction:Article Title: Lon dysfunction-mediated collateral sensitivity drives effective antibiotic combination therapies against tigecycline-resistant pathogens.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BCECF AM Beyotime Cat# S1006 PI Beyotime Cat# ST1569 NPN Aladdin Cat# P110559 Laurdan MCE Cat# HY-D0080 Mag-Fluo-4 AM Maokangbio Cat# MX4544-100UG Fluo-4 AM Beyotime Cat# S1060 SYBR qPCR Master Mix Vazyme Cat# Q312-02 HiScript III RT Supermix for qPCR with gDNA wiper Vazyme Cat# R323-01 Critical commercial assays NAD+/NADH Assay Kit Beyotime Cat# S0175 ROS Detection Assay Kit Beyotime Cat# S0033M Enhanced ATP Assay Kit Beyotime Cat# S0027 Tigecycline ELISA kit MLbio Cat# mL103600 Kanamycin ELISA kit MLbio Cat# mL-D85236 Mouse IL-6 ELISA kit MLbio Cat# mL098430 Mouse TNF-α ELISA kit MLbio Cat# mL002095 Mouse IFN-γ ELISA kit MLbio Cat# mL002277 Mouse IL-4 ELISA kit MLbio Cat# mL064310 Mouse IL-10 ELISA kit MLbio Cat# mL037873 Mouse IL-1β ELISA kit MLbio Cat# mL106733 Cell Counting Kit-8 Beyotime Cat# C0038 LPS (lipopolysaccharide) extraction kit iNtRON Cat# 17141 Chromogenic LAL Endotoxin Assay Kit Beyotime Cat# C0276S FastPure Bacteria DNA Isolation Mini Kit Vazyme Cat# DC103-01 Cycle Pure Kit Omega Cat# D6492 Plasmid Mini Kit II Omega Cat# D6945 EasySpin plus bacterial RNA rapid extraction kit Aidlab Cat# RN4302 ClonExpress Ultra One Step Cloning Kit Vazyme Cat# C115 Deposited data Transcriptome data This study NCBI: PRJNA1253553 Genome sequencing data This study NCBI: PRJNA1252076 Experimental models: Organisms/strains Galleria mellonella Tianjin Huiyude ICR mice Yangzhou University RRID: MGI:2160272 Wistar rats Yangzhou University RRID: MGI:5657554 Oligonucleotides Primers See Tables S9–S12 See Tables S9–S12 Recombinant DNA Plasmid: pUC19 MIAOLING Biology Cat# P0368 Plasmid: pUC19-tet(X4) This study N/A Plasmid: pUC19- tmexCD1-toprJ1 This study N/A Plasmid: pRE112 MIAOLING Biology Cat# P1702 Plasmid: pBAD43 MIAOLING Biology Cat# P1253 Plasmid: pBAD43-lon This study N/A Software and algorithms GraphPad Prism 9 GraphPad Software https://www.graphpad.com Snippy (4.0.2) Snippy Software https://github.com (Continued on next page) Cell Reports 44, 115987, July 22, 2025 19
Detection Assay:Article Title: Lon dysfunction-mediated collateral sensitivity drives effective antibiotic combination therapies against tigecycline-resistant pathogens.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BCECF AM Beyotime Cat# S1006 PI Beyotime Cat# ST1569 NPN Aladdin Cat# P110559 Laurdan MCE Cat# HY-D0080 Mag-Fluo-4 AM Maokangbio Cat# MX4544-100UG Fluo-4 AM Beyotime Cat# S1060 SYBR qPCR Master Mix Vazyme Cat# Q312-02 HiScript III RT Supermix for qPCR with gDNA wiper Vazyme Cat# R323-01 Critical commercial assays NAD+/NADH Assay Kit Beyotime Cat# S0175 ROS Detection Assay Kit Beyotime Cat# S0033M Enhanced ATP Assay Kit Beyotime Cat# S0027 Tigecycline ELISA kit MLbio Cat# mL103600 Kanamycin ELISA kit MLbio Cat# mL-D85236 Mouse IL-6 ELISA kit MLbio Cat# mL098430 Mouse TNF-α ELISA kit MLbio Cat# mL002095 Mouse IFN-γ ELISA kit MLbio Cat# mL002277 Mouse IL-4 ELISA kit MLbio Cat# mL064310 Mouse IL-10 ELISA kit MLbio Cat# mL037873 Mouse IL-1β ELISA kit MLbio Cat# mL106733 Cell Counting Kit-8 Beyotime Cat# C0038 LPS (lipopolysaccharide) extraction kit iNtRON Cat# 17141 Chromogenic LAL Endotoxin Assay Kit Beyotime Cat# C0276S FastPure Bacteria DNA Isolation Mini Kit Vazyme Cat# DC103-01 Cycle Pure Kit Omega Cat# D6492 Plasmid Mini Kit II Omega Cat# D6945 EasySpin plus bacterial RNA rapid extraction kit Aidlab Cat# RN4302 ClonExpress Ultra One Step Cloning Kit Vazyme Cat# C115 Deposited data Transcriptome data This study NCBI: PRJNA1253553 Genome sequencing data This study NCBI: PRJNA1252076 Experimental models: Organisms/strains Galleria mellonella Tianjin Huiyude ICR mice Yangzhou University RRID: MGI:2160272 Wistar rats Yangzhou University RRID: MGI:5657554 Oligonucleotides Primers See Tables S9–S12 See Tables S9–S12 Recombinant DNA Plasmid: pUC19 MIAOLING Biology Cat# P0368 Plasmid: pUC19-tet(X4) This study N/A Plasmid: pUC19- tmexCD1-toprJ1 This study N/A Plasmid: pRE112 MIAOLING Biology Cat# P1702 Plasmid: pBAD43 MIAOLING Biology Cat# P1253 Plasmid: pBAD43-lon This study N/A Software and algorithms GraphPad Prism 9 GraphPad Software https://www.graphpad.com Snippy (4.0.2) Snippy Software https://github.com (Continued on next page) Cell Reports 44, 115987, July 22, 2025 19
ATP Assay:Article Title: Lon dysfunction-mediated collateral sensitivity drives effective antibiotic combination therapies against tigecycline-resistant pathogens.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BCECF AM Beyotime Cat# S1006 PI Beyotime Cat# ST1569 NPN Aladdin Cat# P110559 Laurdan MCE Cat# HY-D0080 Mag-Fluo-4 AM Maokangbio Cat# MX4544-100UG Fluo-4 AM Beyotime Cat# S1060 SYBR qPCR Master Mix Vazyme Cat# Q312-02 HiScript III RT Supermix for qPCR with gDNA wiper Vazyme Cat# R323-01 Critical commercial assays NAD+/NADH Assay Kit Beyotime Cat# S0175 ROS Detection Assay Kit Beyotime Cat# S0033M Enhanced ATP Assay Kit Beyotime Cat# S0027 Tigecycline ELISA kit MLbio Cat# mL103600 Kanamycin ELISA kit MLbio Cat# mL-D85236 Mouse IL-6 ELISA kit MLbio Cat# mL098430 Mouse TNF-α ELISA kit MLbio Cat# mL002095 Mouse IFN-γ ELISA kit MLbio Cat# mL002277 Mouse IL-4 ELISA kit MLbio Cat# mL064310 Mouse IL-10 ELISA kit MLbio Cat# mL037873 Mouse IL-1β ELISA kit MLbio Cat# mL106733 Cell Counting Kit-8 Beyotime Cat# C0038 LPS (lipopolysaccharide) extraction kit iNtRON Cat# 17141 Chromogenic LAL Endotoxin Assay Kit Beyotime Cat# C0276S FastPure Bacteria DNA Isolation Mini Kit Vazyme Cat# DC103-01 Cycle Pure Kit Omega Cat# D6492 Plasmid Mini Kit II Omega Cat# D6945 EasySpin plus bacterial RNA rapid extraction kit Aidlab Cat# RN4302 ClonExpress Ultra One Step Cloning Kit Vazyme Cat# C115 Deposited data Transcriptome data This study NCBI: PRJNA1253553 Genome sequencing data This study NCBI: PRJNA1252076 Experimental models: Organisms/strains Galleria mellonella Tianjin Huiyude ICR mice Yangzhou University RRID: MGI:2160272 Wistar rats Yangzhou University RRID: MGI:5657554 Oligonucleotides Primers See Tables S9–S12 See Tables S9–S12 Recombinant DNA Plasmid: pUC19 MIAOLING Biology Cat# P0368 Plasmid: pUC19-tet(X4) This study N/A Plasmid: pUC19- tmexCD1-toprJ1 This study N/A Plasmid: pRE112 MIAOLING Biology Cat# P1702 Plasmid: pBAD43 MIAOLING Biology Cat# P1253 Plasmid: pBAD43-lon This study N/A Software and algorithms GraphPad Prism 9 GraphPad Software https://www.graphpad.com Snippy (4.0.2) Snippy Software https://github.com (Continued on next page) Cell Reports 44, 115987, July 22, 2025 19
Enzyme-linked Immunosorbent Assay:Article Title: Lon dysfunction-mediated collateral sensitivity drives effective antibiotic combination therapies against tigecycline-resistant pathogens.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BCECF AM Beyotime Cat# S1006 PI Beyotime Cat# ST1569 NPN Aladdin Cat# P110559 Laurdan MCE Cat# HY-D0080 Mag-Fluo-4 AM Maokangbio Cat# MX4544-100UG Fluo-4 AM Beyotime Cat# S1060 SYBR qPCR Master Mix Vazyme Cat# Q312-02 HiScript III RT Supermix for qPCR with gDNA wiper Vazyme Cat# R323-01 Critical commercial assays NAD+/NADH Assay Kit Beyotime Cat# S0175 ROS Detection Assay Kit Beyotime Cat# S0033M Enhanced ATP Assay Kit Beyotime Cat# S0027 Tigecycline ELISA kit MLbio Cat# mL103600 Kanamycin ELISA kit MLbio Cat# mL-D85236 Mouse IL-6 ELISA kit MLbio Cat# mL098430 Mouse TNF-α ELISA kit MLbio Cat# mL002095 Mouse IFN-γ ELISA kit MLbio Cat# mL002277 Mouse IL-4 ELISA kit MLbio Cat# mL064310 Mouse IL-10 ELISA kit MLbio Cat# mL037873 Mouse IL-1β ELISA kit MLbio Cat# mL106733 Cell Counting Kit-8 Beyotime Cat# C0038 LPS (lipopolysaccharide) extraction kit iNtRON Cat# 17141 Chromogenic LAL Endotoxin Assay Kit Beyotime Cat# C0276S FastPure Bacteria DNA Isolation Mini Kit Vazyme Cat# DC103-01 Cycle Pure Kit Omega Cat# D6492 Plasmid Mini Kit II Omega Cat# D6945 EasySpin plus bacterial RNA rapid extraction kit Aidlab Cat# RN4302 ClonExpress Ultra One Step Cloning Kit Vazyme Cat# C115 Deposited data Transcriptome data This study NCBI: PRJNA1253553 Genome sequencing data This study NCBI: PRJNA1252076 Experimental models: Organisms/strains Galleria mellonella Tianjin Huiyude ICR mice Yangzhou University RRID: MGI:2160272 Wistar rats Yangzhou University RRID: MGI:5657554 Oligonucleotides Primers See Tables S9–S12 See Tables S9–S12 Recombinant DNA Plasmid: pUC19 MIAOLING Biology Cat# P0368 Plasmid: pUC19-tet(X4) This study N/A Plasmid: pUC19- tmexCD1-toprJ1 This study N/A Plasmid: pRE112 MIAOLING Biology Cat# P1702 Plasmid: pBAD43 MIAOLING Biology Cat# P1253 Plasmid: pBAD43-lon This study N/A Software and algorithms GraphPad Prism 9 GraphPad Software https://www.graphpad.com Snippy (4.0.2) Snippy Software https://github.com (Continued on next page) Cell Reports 44, 115987, July 22, 2025 19
CCK-8 Assay:Article Title: Lon dysfunction-mediated collateral sensitivity drives effective antibiotic combination therapies against tigecycline-resistant pathogens.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BCECF AM Beyotime Cat# S1006 PI Beyotime Cat# ST1569 NPN Aladdin Cat# P110559 Laurdan MCE Cat# HY-D0080 Mag-Fluo-4 AM Maokangbio Cat# MX4544-100UG Fluo-4 AM Beyotime Cat# S1060 SYBR qPCR Master Mix Vazyme Cat# Q312-02 HiScript III RT Supermix for qPCR with gDNA wiper Vazyme Cat# R323-01 Critical commercial assays NAD+/NADH Assay Kit Beyotime Cat# S0175 ROS Detection Assay Kit Beyotime Cat# S0033M Enhanced ATP Assay Kit Beyotime Cat# S0027 Tigecycline ELISA kit MLbio Cat# mL103600 Kanamycin ELISA kit MLbio Cat# mL-D85236 Mouse IL-6 ELISA kit MLbio Cat# mL098430 Mouse TNF-α ELISA kit MLbio Cat# mL002095 Mouse IFN-γ ELISA kit MLbio Cat# mL002277 Mouse IL-4 ELISA kit MLbio Cat# mL064310 Mouse IL-10 ELISA kit MLbio Cat# mL037873 Mouse IL-1β ELISA kit MLbio Cat# mL106733 Cell Counting Kit-8 Beyotime Cat# C0038 LPS (lipopolysaccharide) extraction kit iNtRON Cat# 17141 Chromogenic LAL Endotoxin Assay Kit Beyotime Cat# C0276S FastPure Bacteria DNA Isolation Mini Kit Vazyme Cat# DC103-01 Cycle Pure Kit Omega Cat# D6492 Plasmid Mini Kit II Omega Cat# D6945 EasySpin plus bacterial RNA rapid extraction kit Aidlab Cat# RN4302 ClonExpress Ultra One Step Cloning Kit Vazyme Cat# C115 Deposited data Transcriptome data This study NCBI: PRJNA1253553 Genome sequencing data This study NCBI: PRJNA1252076 Experimental models: Organisms/strains Galleria mellonella Tianjin Huiyude ICR mice Yangzhou University RRID: MGI:2160272 Wistar rats Yangzhou University RRID: MGI:5657554 Oligonucleotides Primers See Tables S9–S12 See Tables S9–S12 Recombinant DNA Plasmid: pUC19 MIAOLING Biology Cat# P0368 Plasmid: pUC19-tet(X4) This study N/A Plasmid: pUC19- tmexCD1-toprJ1 This study N/A Plasmid: pRE112 MIAOLING Biology Cat# P1702 Plasmid: pBAD43 MIAOLING Biology Cat# P1253 Plasmid: pBAD43-lon This study N/A Software and algorithms GraphPad Prism 9 GraphPad Software https://www.graphpad.com Snippy (4.0.2) Snippy Software https://github.com (Continued on next page) Cell Reports 44, 115987, July 22, 2025 19
Extraction:Article Title: Lon dysfunction-mediated collateral sensitivity drives effective antibiotic combination therapies against tigecycline-resistant pathogens.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BCECF AM Beyotime Cat# S1006 PI Beyotime Cat# ST1569 NPN Aladdin Cat# P110559 Laurdan MCE Cat# HY-D0080 Mag-Fluo-4 AM Maokangbio Cat# MX4544-100UG Fluo-4 AM Beyotime Cat# S1060 SYBR qPCR Master Mix Vazyme Cat# Q312-02 HiScript III RT Supermix for qPCR with gDNA wiper Vazyme Cat# R323-01 Critical commercial assays NAD+/NADH Assay Kit Beyotime Cat# S0175 ROS Detection Assay Kit Beyotime Cat# S0033M Enhanced ATP Assay Kit Beyotime Cat# S0027 Tigecycline ELISA kit MLbio Cat# mL103600 Kanamycin ELISA kit MLbio Cat# mL-D85236 Mouse IL-6 ELISA kit MLbio Cat# mL098430 Mouse TNF-α ELISA kit MLbio Cat# mL002095 Mouse IFN-γ ELISA kit MLbio Cat# mL002277 Mouse IL-4 ELISA kit MLbio Cat# mL064310 Mouse IL-10 ELISA kit MLbio Cat# mL037873 Mouse IL-1β ELISA kit MLbio Cat# mL106733 Cell Counting Kit-8 Beyotime Cat# C0038 LPS (lipopolysaccharide) extraction kit iNtRON Cat# 17141 Chromogenic LAL Endotoxin Assay Kit Beyotime Cat# C0276S FastPure Bacteria DNA Isolation Mini Kit Vazyme Cat# DC103-01 Cycle Pure Kit Omega Cat# D6492 Plasmid Mini Kit II Omega Cat# D6945 EasySpin plus bacterial RNA rapid extraction kit Aidlab Cat# RN4302 ClonExpress Ultra One Step Cloning Kit Vazyme Cat# C115 Deposited data Transcriptome data This study NCBI: PRJNA1253553 Genome sequencing data This study NCBI: PRJNA1252076 Experimental models: Organisms/strains Galleria mellonella Tianjin Huiyude ICR mice Yangzhou University RRID: MGI:2160272 Wistar rats Yangzhou University RRID: MGI:5657554 Oligonucleotides Primers See Tables S9–S12 See Tables S9–S12 Recombinant DNA Plasmid: pUC19 MIAOLING Biology Cat# P0368 Plasmid: pUC19-tet(X4) This study N/A Plasmid: pUC19- tmexCD1-toprJ1 This study N/A Plasmid: pRE112 MIAOLING Biology Cat# P1702 Plasmid: pBAD43 MIAOLING Biology Cat# P1253 Plasmid: pBAD43-lon This study N/A Software and algorithms GraphPad Prism 9 GraphPad Software https://www.graphpad.com Snippy (4.0.2) Snippy Software https://github.com (Continued on next page) Cell Reports 44, 115987, July 22, 2025 19
Endotoxin Assay:Article Title: Lon dysfunction-mediated collateral sensitivity drives effective antibiotic combination therapies against tigecycline-resistant pathogens.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BCECF AM Beyotime Cat# S1006 PI Beyotime Cat# ST1569 NPN Aladdin Cat# P110559 Laurdan MCE Cat# HY-D0080 Mag-Fluo-4 AM Maokangbio Cat# MX4544-100UG Fluo-4 AM Beyotime Cat# S1060 SYBR qPCR Master Mix Vazyme Cat# Q312-02 HiScript III RT Supermix for qPCR with gDNA wiper Vazyme Cat# R323-01 Critical commercial assays NAD+/NADH Assay Kit Beyotime Cat# S0175 ROS Detection Assay Kit Beyotime Cat# S0033M Enhanced ATP Assay Kit Beyotime Cat# S0027 Tigecycline ELISA kit MLbio Cat# mL103600 Kanamycin ELISA kit MLbio Cat# mL-D85236 Mouse IL-6 ELISA kit MLbio Cat# mL098430 Mouse TNF-α ELISA kit MLbio Cat# mL002095 Mouse IFN-γ ELISA kit MLbio Cat# mL002277 Mouse IL-4 ELISA kit MLbio Cat# mL064310 Mouse IL-10 ELISA kit MLbio Cat# mL037873 Mouse IL-1β ELISA kit MLbio Cat# mL106733 Cell Counting Kit-8 Beyotime Cat# C0038 LPS (lipopolysaccharide) extraction kit iNtRON Cat# 17141 Chromogenic LAL Endotoxin Assay Kit Beyotime Cat# C0276S FastPure Bacteria DNA Isolation Mini Kit Vazyme Cat# DC103-01 Cycle Pure Kit Omega Cat# D6492 Plasmid Mini Kit II Omega Cat# D6945 EasySpin plus bacterial RNA rapid extraction kit Aidlab Cat# RN4302 ClonExpress Ultra One Step Cloning Kit Vazyme Cat# C115 Deposited data Transcriptome data This study NCBI: PRJNA1253553 Genome sequencing data This study NCBI: PRJNA1252076 Experimental models: Organisms/strains Galleria mellonella Tianjin Huiyude ICR mice Yangzhou University RRID: MGI:2160272 Wistar rats Yangzhou University RRID: MGI:5657554 Oligonucleotides Primers See Tables S9–S12 See Tables S9–S12 Recombinant DNA Plasmid: pUC19 MIAOLING Biology Cat# P0368 Plasmid: pUC19-tet(X4) This study N/A Plasmid: pUC19- tmexCD1-toprJ1 This study N/A Plasmid: pRE112 MIAOLING Biology Cat# P1702 Plasmid: pBAD43 MIAOLING Biology Cat# P1253 Plasmid: pBAD43-lon This study N/A Software and algorithms GraphPad Prism 9 GraphPad Software https://www.graphpad.com Snippy (4.0.2) Snippy Software https://github.com (Continued on next page) Cell Reports 44, 115987, July 22, 2025 19
Bacteria:Article Title: Lon dysfunction-mediated collateral sensitivity drives effective antibiotic combination therapies against tigecycline-resistant pathogens.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BCECF AM Beyotime Cat# S1006 PI Beyotime Cat# ST1569 NPN Aladdin Cat# P110559 Laurdan MCE Cat# HY-D0080 Mag-Fluo-4 AM Maokangbio Cat# MX4544-100UG Fluo-4 AM Beyotime Cat# S1060 SYBR qPCR Master Mix Vazyme Cat# Q312-02 HiScript III RT Supermix for qPCR with gDNA wiper Vazyme Cat# R323-01 Critical commercial assays NAD+/NADH Assay Kit Beyotime Cat# S0175 ROS Detection Assay Kit Beyotime Cat# S0033M Enhanced ATP Assay Kit Beyotime Cat# S0027 Tigecycline ELISA kit MLbio Cat# mL103600 Kanamycin ELISA kit MLbio Cat# mL-D85236 Mouse IL-6 ELISA kit MLbio Cat# mL098430 Mouse TNF-α ELISA kit MLbio Cat# mL002095 Mouse IFN-γ ELISA kit MLbio Cat# mL002277 Mouse IL-4 ELISA kit MLbio Cat# mL064310 Mouse IL-10 ELISA kit MLbio Cat# mL037873 Mouse IL-1β ELISA kit MLbio Cat# mL106733 Cell Counting Kit-8 Beyotime Cat# C0038 LPS (lipopolysaccharide) extraction kit iNtRON Cat# 17141 Chromogenic LAL Endotoxin Assay Kit Beyotime Cat# C0276S FastPure Bacteria DNA Isolation Mini Kit Vazyme Cat# DC103-01 Cycle Pure Kit Omega Cat# D6492 Plasmid Mini Kit II Omega Cat# D6945 EasySpin plus bacterial RNA rapid extraction kit Aidlab Cat# RN4302 ClonExpress Ultra One Step Cloning Kit Vazyme Cat# C115 Deposited data Transcriptome data This study NCBI: PRJNA1253553 Genome sequencing data This study NCBI: PRJNA1252076 Experimental models: Organisms/strains Galleria mellonella Tianjin Huiyude ICR mice Yangzhou University RRID: MGI:2160272 Wistar rats Yangzhou University RRID: MGI:5657554 Oligonucleotides Primers See Tables S9–S12 See Tables S9–S12 Recombinant DNA Plasmid: pUC19 MIAOLING Biology Cat# P0368 Plasmid: pUC19-tet(X4) This study N/A Plasmid: pUC19- tmexCD1-toprJ1 This study N/A Plasmid: pRE112 MIAOLING Biology Cat# P1702 Plasmid: pBAD43 MIAOLING Biology Cat# P1253 Plasmid: pBAD43-lon This study N/A Software and algorithms GraphPad Prism 9 GraphPad Software https://www.graphpad.com Snippy (4.0.2) Snippy Software https://github.com (Continued on next page) Cell Reports 44, 115987, July 22, 2025 19
DNA Extraction:Article Title: Lon dysfunction-mediated collateral sensitivity drives effective antibiotic combination therapies against tigecycline-resistant pathogens.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BCECF AM Beyotime Cat# S1006 PI Beyotime Cat# ST1569 NPN Aladdin Cat# P110559 Laurdan MCE Cat# HY-D0080 Mag-Fluo-4 AM Maokangbio Cat# MX4544-100UG Fluo-4 AM Beyotime Cat# S1060 SYBR qPCR Master Mix Vazyme Cat# Q312-02 HiScript III RT Supermix for qPCR with gDNA wiper Vazyme Cat# R323-01 Critical commercial assays NAD+/NADH Assay Kit Beyotime Cat# S0175 ROS Detection Assay Kit Beyotime Cat# S0033M Enhanced ATP Assay Kit Beyotime Cat# S0027 Tigecycline ELISA kit MLbio Cat# mL103600 Kanamycin ELISA kit MLbio Cat# mL-D85236 Mouse IL-6 ELISA kit MLbio Cat# mL098430 Mouse TNF-α ELISA kit MLbio Cat# mL002095 Mouse IFN-γ ELISA kit MLbio Cat# mL002277 Mouse IL-4 ELISA kit MLbio Cat# mL064310 Mouse IL-10 ELISA kit MLbio Cat# mL037873 Mouse IL-1β ELISA kit MLbio Cat# mL106733 Cell Counting Kit-8 Beyotime Cat# C0038 LPS (lipopolysaccharide) extraction kit iNtRON Cat# 17141 Chromogenic LAL Endotoxin Assay Kit Beyotime Cat# C0276S FastPure Bacteria DNA Isolation Mini Kit Vazyme Cat# DC103-01 Cycle Pure Kit Omega Cat# D6492 Plasmid Mini Kit II Omega Cat# D6945 EasySpin plus bacterial RNA rapid extraction kit Aidlab Cat# RN4302 ClonExpress Ultra One Step Cloning Kit Vazyme Cat# C115 Deposited data Transcriptome data This study NCBI: PRJNA1253553 Genome sequencing data This study NCBI: PRJNA1252076 Experimental models: Organisms/strains Galleria mellonella Tianjin Huiyude ICR mice Yangzhou University RRID: MGI:2160272 Wistar rats Yangzhou University RRID: MGI:5657554 Oligonucleotides Primers See Tables S9–S12 See Tables S9–S12 Recombinant DNA Plasmid: pUC19 MIAOLING Biology Cat# P0368 Plasmid: pUC19-tet(X4) This study N/A Plasmid: pUC19- tmexCD1-toprJ1 This study N/A Plasmid: pRE112 MIAOLING Biology Cat# P1702 Plasmid: pBAD43 MIAOLING Biology Cat# P1253 Plasmid: pBAD43-lon This study N/A Software and algorithms GraphPad Prism 9 GraphPad Software https://www.graphpad.com Snippy (4.0.2) Snippy Software https://github.com (Continued on next page) Cell Reports 44, 115987, July 22, 2025 19
Plasmid Preparation:Article Title: Lon dysfunction-mediated collateral sensitivity drives effective antibiotic combination therapies against tigecycline-resistant pathogens.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BCECF AM Beyotime Cat# S1006 PI Beyotime Cat# ST1569 NPN Aladdin Cat# P110559 Laurdan MCE Cat# HY-D0080 Mag-Fluo-4 AM Maokangbio Cat# MX4544-100UG Fluo-4 AM Beyotime Cat# S1060 SYBR qPCR Master Mix Vazyme Cat# Q312-02 HiScript III RT Supermix for qPCR with gDNA wiper Vazyme Cat# R323-01 Critical commercial assays NAD+/NADH Assay Kit Beyotime Cat# S0175 ROS Detection Assay Kit Beyotime Cat# S0033M Enhanced ATP Assay Kit Beyotime Cat# S0027 Tigecycline ELISA kit MLbio Cat# mL103600 Kanamycin ELISA kit MLbio Cat# mL-D85236 Mouse IL-6 ELISA kit MLbio Cat# mL098430 Mouse TNF-α ELISA kit MLbio Cat# mL002095 Mouse IFN-γ ELISA kit MLbio Cat# mL002277 Mouse IL-4 ELISA kit MLbio Cat# mL064310 Mouse IL-10 ELISA kit MLbio Cat# mL037873 Mouse IL-1β ELISA kit MLbio Cat# mL106733 Cell Counting Kit-8 Beyotime Cat# C0038 LPS (lipopolysaccharide) extraction kit iNtRON Cat# 17141 Chromogenic LAL Endotoxin Assay Kit Beyotime Cat# C0276S FastPure Bacteria DNA Isolation Mini Kit Vazyme Cat# DC103-01 Cycle Pure Kit Omega Cat# D6492 Plasmid Mini Kit II Omega Cat# D6945 EasySpin plus bacterial RNA rapid extraction kit Aidlab Cat# RN4302 ClonExpress Ultra One Step Cloning Kit Vazyme Cat# C115 Deposited data Transcriptome data This study NCBI: PRJNA1253553 Genome sequencing data This study NCBI: PRJNA1252076 Experimental models: Organisms/strains Galleria mellonella Tianjin Huiyude ICR mice Yangzhou University RRID: MGI:2160272 Wistar rats Yangzhou University RRID: MGI:5657554 Oligonucleotides Primers See Tables S9–S12 See Tables S9–S12 Recombinant DNA Plasmid: pUC19 MIAOLING Biology Cat# P0368 Plasmid: pUC19-tet(X4) This study N/A Plasmid: pUC19- tmexCD1-toprJ1 This study N/A Plasmid: pRE112 MIAOLING Biology Cat# P1702 Plasmid: pBAD43 MIAOLING Biology Cat# P1253 Plasmid: pBAD43-lon This study N/A Software and algorithms GraphPad Prism 9 GraphPad Software https://www.graphpad.com Snippy (4.0.2) Snippy Software https://github.com (Continued on next page) Cell Reports 44, 115987, July 22, 2025 19
Cloning:Article Title: Lon dysfunction-mediated collateral sensitivity drives effective antibiotic combination therapies against tigecycline-resistant pathogens.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BCECF AM Beyotime Cat# S1006 PI Beyotime Cat# ST1569 NPN Aladdin Cat# P110559 Laurdan MCE Cat# HY-D0080 Mag-Fluo-4 AM Maokangbio Cat# MX4544-100UG Fluo-4 AM Beyotime Cat# S1060 SYBR qPCR Master Mix Vazyme Cat# Q312-02 HiScript III RT Supermix for qPCR with gDNA wiper Vazyme Cat# R323-01 Critical commercial assays NAD+/NADH Assay Kit Beyotime Cat# S0175 ROS Detection Assay Kit Beyotime Cat# S0033M Enhanced ATP Assay Kit Beyotime Cat# S0027 Tigecycline ELISA kit MLbio Cat# mL103600 Kanamycin ELISA kit MLbio Cat# mL-D85236 Mouse IL-6 ELISA kit MLbio Cat# mL098430 Mouse TNF-α ELISA kit MLbio Cat# mL002095 Mouse IFN-γ ELISA kit MLbio Cat# mL002277 Mouse IL-4 ELISA kit MLbio Cat# mL064310 Mouse IL-10 ELISA kit MLbio Cat# mL037873 Mouse IL-1β ELISA kit MLbio Cat# mL106733 Cell Counting Kit-8 Beyotime Cat# C0038 LPS (lipopolysaccharide) extraction kit iNtRON Cat# 17141 Chromogenic LAL Endotoxin Assay Kit Beyotime Cat# C0276S FastPure Bacteria DNA Isolation Mini Kit Vazyme Cat# DC103-01 Cycle Pure Kit Omega Cat# D6492 Plasmid Mini Kit II Omega Cat# D6945 EasySpin plus bacterial RNA rapid extraction kit Aidlab Cat# RN4302 ClonExpress Ultra One Step Cloning Kit Vazyme Cat# C115 Deposited data Transcriptome data This study NCBI: PRJNA1253553 Genome sequencing data This study NCBI: PRJNA1252076 Experimental models: Organisms/strains Galleria mellonella Tianjin Huiyude ICR mice Yangzhou University RRID: MGI:2160272 Wistar rats Yangzhou University RRID: MGI:5657554 Oligonucleotides Primers See Tables S9–S12 See Tables S9–S12 Recombinant DNA Plasmid: pUC19 MIAOLING Biology Cat# P0368 Plasmid: pUC19-tet(X4) This study N/A Plasmid: pUC19- tmexCD1-toprJ1 This study N/A Plasmid: pRE112 MIAOLING Biology Cat# P1702 Plasmid: pBAD43 MIAOLING Biology Cat# P1253 Plasmid: pBAD43-lon This study N/A Software and algorithms GraphPad Prism 9 GraphPad Software https://www.graphpad.com Snippy (4.0.2) Snippy Software https://github.com (Continued on next page) Cell Reports 44, 115987, July 22, 2025 19
Sequencing:Article Title: Lon dysfunction-mediated collateral sensitivity drives effective antibiotic combination therapies against tigecycline-resistant pathogens.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BCECF AM Beyotime Cat# S1006 PI Beyotime Cat# ST1569 NPN Aladdin Cat# P110559 Laurdan MCE Cat# HY-D0080 Mag-Fluo-4 AM Maokangbio Cat# MX4544-100UG Fluo-4 AM Beyotime Cat# S1060 SYBR qPCR Master Mix Vazyme Cat# Q312-02 HiScript III RT Supermix for qPCR with gDNA wiper Vazyme Cat# R323-01 Critical commercial assays NAD+/NADH Assay Kit Beyotime Cat# S0175 ROS Detection Assay Kit Beyotime Cat# S0033M Enhanced ATP Assay Kit Beyotime Cat# S0027 Tigecycline ELISA kit MLbio Cat# mL103600 Kanamycin ELISA kit MLbio Cat# mL-D85236 Mouse IL-6 ELISA kit MLbio Cat# mL098430 Mouse TNF-α ELISA kit MLbio Cat# mL002095 Mouse IFN-γ ELISA kit MLbio Cat# mL002277 Mouse IL-4 ELISA kit MLbio Cat# mL064310 Mouse IL-10 ELISA kit MLbio Cat# mL037873 Mouse IL-1β ELISA kit MLbio Cat# mL106733 Cell Counting Kit-8 Beyotime Cat# C0038 LPS (lipopolysaccharide) extraction kit iNtRON Cat# 17141 Chromogenic LAL Endotoxin Assay Kit Beyotime Cat# C0276S FastPure Bacteria DNA Isolation Mini Kit Vazyme Cat# DC103-01 Cycle Pure Kit Omega Cat# D6492 Plasmid Mini Kit II Omega Cat# D6945 EasySpin plus bacterial RNA rapid extraction kit Aidlab Cat# RN4302 ClonExpress Ultra One Step Cloning Kit Vazyme Cat# C115 Deposited data Transcriptome data This study NCBI: PRJNA1253553 Genome sequencing data This study NCBI: PRJNA1252076 Experimental models: Organisms/strains Galleria mellonella Tianjin Huiyude ICR mice Yangzhou University RRID: MGI:2160272 Wistar rats Yangzhou University RRID: MGI:5657554 Oligonucleotides Primers See Tables S9–S12 See Tables S9–S12 Recombinant DNA Plasmid: pUC19 MIAOLING Biology Cat# P0368 Plasmid: pUC19-tet(X4) This study N/A Plasmid: pUC19- tmexCD1-toprJ1 This study N/A Plasmid: pRE112 MIAOLING Biology Cat# P1702 Plasmid: pBAD43 MIAOLING Biology Cat# P1253 Plasmid: pBAD43-lon This study N/A Software and algorithms GraphPad Prism 9 GraphPad Software https://www.graphpad.com Snippy (4.0.2) Snippy Software https://github.com (Continued on next page) Cell Reports 44, 115987, July 22, 2025 19
Recombinant:Article Title: Lon dysfunction-mediated collateral sensitivity drives effective antibiotic combination therapies against tigecycline-resistant pathogens.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BCECF AM Beyotime Cat# S1006 PI Beyotime Cat# ST1569 NPN Aladdin Cat# P110559 Laurdan MCE Cat# HY-D0080 Mag-Fluo-4 AM Maokangbio Cat# MX4544-100UG Fluo-4 AM Beyotime Cat# S1060 SYBR qPCR Master Mix Vazyme Cat# Q312-02 HiScript III RT Supermix for qPCR with gDNA wiper Vazyme Cat# R323-01 Critical commercial assays NAD+/NADH Assay Kit Beyotime Cat# S0175 ROS Detection Assay Kit Beyotime Cat# S0033M Enhanced ATP Assay Kit Beyotime Cat# S0027 Tigecycline ELISA kit MLbio Cat# mL103600 Kanamycin ELISA kit MLbio Cat# mL-D85236 Mouse IL-6 ELISA kit MLbio Cat# mL098430 Mouse TNF-α ELISA kit MLbio Cat# mL002095 Mouse IFN-γ ELISA kit MLbio Cat# mL002277 Mouse IL-4 ELISA kit MLbio Cat# mL064310 Mouse IL-10 ELISA kit MLbio Cat# mL037873 Mouse IL-1β ELISA kit MLbio Cat# mL106733 Cell Counting Kit-8 Beyotime Cat# C0038 LPS (lipopolysaccharide) extraction kit iNtRON Cat# 17141 Chromogenic LAL Endotoxin Assay Kit Beyotime Cat# C0276S FastPure Bacteria DNA Isolation Mini Kit Vazyme Cat# DC103-01 Cycle Pure Kit Omega Cat# D6492 Plasmid Mini Kit II Omega Cat# D6945 EasySpin plus bacterial RNA rapid extraction kit Aidlab Cat# RN4302 ClonExpress Ultra One Step Cloning Kit Vazyme Cat# C115 Deposited data Transcriptome data This study NCBI: PRJNA1253553 Genome sequencing data This study NCBI: PRJNA1252076 Experimental models: Organisms/strains Galleria mellonella Tianjin Huiyude ICR mice Yangzhou University RRID: MGI:2160272 Wistar rats Yangzhou University RRID: MGI:5657554 Oligonucleotides Primers See Tables S9–S12 See Tables S9–S12 Recombinant DNA Plasmid: pUC19 MIAOLING Biology Cat# P0368 Plasmid: pUC19-tet(X4) This study N/A Plasmid: pUC19- tmexCD1-toprJ1 This study N/A Plasmid: pRE112 MIAOLING Biology Cat# P1702 Plasmid: pBAD43 MIAOLING Biology Cat# P1253 Plasmid: pBAD43-lon This study N/A Software and algorithms GraphPad Prism 9 GraphPad Software https://www.graphpad.com Snippy (4.0.2) Snippy Software https://github.com (Continued on next page) Cell Reports 44, 115987, July 22, 2025 19
Software:Article Title: Lon dysfunction-mediated collateral sensitivity drives effective antibiotic combination therapies against tigecycline-resistant pathogens.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BCECF AM Beyotime Cat# S1006 PI Beyotime Cat# ST1569 NPN Aladdin Cat# P110559 Laurdan MCE Cat# HY-D0080 Mag-Fluo-4 AM Maokangbio Cat# MX4544-100UG Fluo-4 AM Beyotime Cat# S1060 SYBR qPCR Master Mix Vazyme Cat# Q312-02 HiScript III RT Supermix for qPCR with gDNA wiper Vazyme Cat# R323-01 Critical commercial assays NAD+/NADH Assay Kit Beyotime Cat# S0175 ROS Detection Assay Kit Beyotime Cat# S0033M Enhanced ATP Assay Kit Beyotime Cat# S0027 Tigecycline ELISA kit MLbio Cat# mL103600 Kanamycin ELISA kit MLbio Cat# mL-D85236 Mouse IL-6 ELISA kit MLbio Cat# mL098430 Mouse TNF-α ELISA kit MLbio Cat# mL002095 Mouse IFN-γ ELISA kit MLbio Cat# mL002277 Mouse IL-4 ELISA kit MLbio Cat# mL064310 Mouse IL-10 ELISA kit MLbio Cat# mL037873 Mouse IL-1β ELISA kit MLbio Cat# mL106733 Cell Counting Kit-8 Beyotime Cat# C0038 LPS (lipopolysaccharide) extraction kit iNtRON Cat# 17141 Chromogenic LAL Endotoxin Assay Kit Beyotime Cat# C0276S FastPure Bacteria DNA Isolation Mini Kit Vazyme Cat# DC103-01 Cycle Pure Kit Omega Cat# D6492 Plasmid Mini Kit II Omega Cat# D6945 EasySpin plus bacterial RNA rapid extraction kit Aidlab Cat# RN4302 ClonExpress Ultra One Step Cloning Kit Vazyme Cat# C115 Deposited data Transcriptome data This study NCBI: PRJNA1253553 Genome sequencing data This study NCBI: PRJNA1252076 Experimental models: Organisms/strains Galleria mellonella Tianjin Huiyude ICR mice Yangzhou University RRID: MGI:2160272 Wistar rats Yangzhou University RRID: MGI:5657554 Oligonucleotides Primers See Tables S9–S12 See Tables S9–S12 Recombinant DNA Plasmid: pUC19 MIAOLING Biology Cat# P0368 Plasmid: pUC19-tet(X4) This study N/A Plasmid: pUC19- tmexCD1-toprJ1 This study N/A Plasmid: pRE112 MIAOLING Biology Cat# P1702 Plasmid: pBAD43 MIAOLING Biology Cat# P1253 Plasmid: pBAD43-lon This study N/A Software and algorithms GraphPad Prism 9 GraphPad Software https://www.graphpad.com Snippy (4.0.2) Snippy Software https://github.com (Continued on next page) Cell Reports 44, 115987, July 22, 2025 19
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